Laboratory Fee Schedule
Procedure: MBP0229A
CPT: 87389, 86701, 86702, 87390
| Synonym(s): |
HIV Antigen/Antibody, HIV-1, HIV-2, p24 antigen, Human Immunodeficiency Virus, HIV-1/-2 Ag-Ab |
| Requisition Form |
G-2A |
| Test Description |
The BioPlex 2200 HIV Ag-Ab assay is a multiplex flow immunoassay that is run on the fully automated BioPlex 2200 system. The assay methodology permits simultaneous detection and identification of anti-HIV-1, anti-HIV-2, and HIV-1 p24 antigen in a single reaction vessel. |
| Pre-Approval Needed |
N/A |
| Supplemental Information Required |
N/A |
| Supplemental Form(s) |
N/A |
| Performed on Specimens from (sources) |
Human |
| Sample/Specimen Type for Testing |
Serum collected in serum separator tube (SST) or red top tubes |
| Minimum Volume/Size Required |
1.0 mL |
| Storage/Preservation Prior to Shipping |
- Store at 2°C to 8°C for up to 5 days.
- Store frozen at -20°C or colder for long term storage.
|
| Transport Medium |
N/A |
| Specimen Labeling |
- Two patient-specific identifiers required on specimen (e.g., patient full name, date of birth, medical record number)
- Three patient identifiers are preferred.
- Patient identifiers on specimen label must exactly match identifiers on submission form (e.g., abbreviated name on label and full name on form is not acceptable.)
- Additional DSHS Laboratory specimen labeling guidelines available online here.
|
| Shipping and Specimen Handling Requirements |
- Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
- Category B Biological Substance, UN3373 shipping requirements apply.
- Handle as infectious agent using universal precautions.
- Triple contain in accordance with federal shipping regulations for infectious agents.
- Submitters are responsible for packaging and shipping specimens according to regulatory requirements.
- If specimens will arrive at the Lab within 5 days of collection, refrigerate at 2°C –8°C and ship overnight on cold packs.
- If specimens will arrive at the Lab more than 5 days after date of collection, freeze at -20°C or below, and ship overnight on dry ice.
- Additional DSHS Laboratory specimen shipping guidelines online here.
|
| Method |
Multiplex Flow Immunoassay |
| Turn-around Time |
4 working days |
| Interferences/Limitations |
Contaminated, icteric, lipemic, hemolyzed or heat inactivated sera may cause erroneous results and should be avoided. |
| Common Causes for Rejection |
- Insufficient specimen quantity.
- Unacceptable specimen type or source.
- Improper shipping conditions.
- Expired media or collection container.
- Expired reagents/media/collection container.
- Discrepancies between G-2A submission form and specimen label.
- Missing/incomplete/illegible submission form or label.
|
| Additional Information |
- This assay should not be used on individuals younger than 2 years of age.
- Non-reactive results do not preclude the possibility of exposure to or infection with HIV-1 and/or HIV-2
- Reactive specimens will automatically reflex to HIV Confirmation testing per the CDC algorithm.
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