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PCR: Ebola Zaire

Laboratory Fee Schedule

Procedure #: BTA0039

CPT: 87798

Synonym(s): Ebola Zaire Real-Time PCR
Requisition Form G-27A
Test Description Detection of Ebola Zaire RNA by Real-Time PCR
Pre-Approval Needed

DSHS Epidemiology consultation with physician.

Must notify BioThreat Team prior to sample submission: (512) 689-5537

Supplemental Information Required N/A
Supplemental Form(s) N/A
Performed on Specimens from (sources) Human
Sample/Specimen Type for Testing
  • Whole blood

  • Serum

  • Plasma

  • Urine (with paired blood sample only)

Minimum Volume/Size Required 1 ml for fluids
Storage/Preservation Prior to Shipping

Clinical samples

  • 2-8°C (cold)

  • Can be stored up to 7 days

Serum and plasma

  • May be frozen at <-70°C if a delay is anticipated

Transport Medium Purple top EDTA tubes for blood
Specimen Labeling
  • Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)

  • Identifiers on specimen must exactly match submission form.

Shipping and Specimen Handling Requirements
  • Ship according to Dangerous Good Regulations, IATA, and/or CFR 49.

  • Handled as infectious agent using universal precautions.

  • Triple contained in accordance with federal shipping regulations for infectious agents.

  • See also Emergency Preparedness resources online.

Notify BioThreat Team prior to sample submission: (512) 689-5537

Method Real-Time PCR
Turn-around Time 1 day after receipt
Interferences/Limitations Improper storage or shipping temperatures may limit organism recovery.
Common Causes for Rejection
  • Insufficient quantity

  • Unacceptable specimen type or source

  • Improper shipping conditions

  • Expired media or collection container

  • Discrepancies between specimen label and submission form

  • Incomplete or missing submission form

Additional Information N/A