WhoopingCoughID

Bordetella pertussis identification 

Laboratory Fee Schedule

Procedure: MAA0028A 

CPT: 87077


Bordetella pertussis identification
Synonym(s): Whooping cough
Requisition Form G-2B
Test Description Identification of Bordetella pertussis from clinical specimens or isolate
Pre-Approval Needed N/A
Supplemental Information Required N/A
Supplemental Form(s) N/A
Performed on Specimens from (sources) Human
Sample/Specimen Type for Testing
  • Clinical sample
  • Pure isolate
Minimum Volume/Size Required

At least one viable colony

Storage/Preservation Prior to Shipping

Clinical  

  • Nasopharyngeal  swabs: collect on Dacron or calcium alginate swabs only  

Isolates 

  • Isolates can be frozen in cryopreservation medium or refrigerated on Regan-Lowe, Bordet Gengou or Charcoal agar.   
Transport Medium

Clinical

  • Regan-Lowe and Amies with charcoal 

Isolate

  • Regan-Lowe, Bordet Gengou, Charcoal Agar, or cryopreservation medium
Specimen Labeling
  • Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)
  • Identifiers on specimen must exactly match submission form.
Shipping and Specimen Handling Requirements
  • Ship according to Dangerous Good Regulations, IATA, and/or CFR 49. 

Clinical

  • If transit time to laboratory will be >24 hours, use Regan-Lowe transport medium and ship on cold pack. 
  • If transit time to laboratory will be ≤24 hours, Amies medium with charcoal or Regan-Lowe transport medium may be used. Ship at on cold pack

Isolates 

  • Isolates should be shipped refrigerated 2 to 8°C as soon as possible between 24-48 hours on Regan-Lowe media or transport media.  Frozen isolates should be shipped on dry ice. 
Method Bacterial culture
Turn-around Time

Negative: 10 days 

Positive: 5-7 days

Interferences/Limitations
  • Material must be viable. Improper storage or shipping temperatures limit organism recovery.
  • Prior antibiotic treatment will adversely affect results and patients coughing more than two weeks will likely not be culture positive.
Common Causes for Rejection
  • Insufficient quantity
  • Unacceptable specimen type or source
  • Improper shipping conditions
  • Expired media or collection container
  • Discrepancies between specimen label and submission form
  • Incomplete or missing submission form. 
Additional Information Culture Isolate will be confirmed by PCR

Last updated July 8, 2020