| Synonym(s): |
Arbovirus, Flavivirus |
| Requisition Form |
G-2V |
| Test Description |
IgM capture ELISA is for the qualitative detection of IgM antibodies to Dengue virus in serum for the presumptive diagnosis of Dengue fever. |
| Pre-Approval Needed |
N/A |
| Supplemental Information Required |
N/A |
| Supplemental Form(s) |
N/A |
| Performed on Specimens from (sources) |
Human |
| Sample/Specimen Type for Testing |
Serum collected in Serum Separator tubes (SST) or red top tubes. |
| Minimum Volume/Size Required |
1.0 mL. |
| Storage/Preservation Prior to Shipping |
Serum separated from the clot may be stored at 2°C to 8°Cup to 48 hours, but then must be frozen at -20°C or colder until used.
|
| Transport Medium |
N/A |
| Specimen Labeling |
- At least two patient-specific identifiers required (e.g., patient full name, date of birth, Medical Record number)
- Three patient identifiers are preferred.
- Identifiers on specimen must exactly match submission form.
- Additional DSHS Laboratory specimen labeling guidelines available online here.
|
| Shipping and Specimen Handling Requirements |
- Ship according to Dangerous Goods Regulations, IATA, and/or CFR 49.
- Handle as infectious agent using universal precautions.
- Triple contain in accordance with federal shipping regulations for infectious agents.
Additional forms & resources
- If specimens will arrive at the lab within 48 hours of collection, refrigerate at 2°C - 8°and ship overnight on cold packs.
- If specimens will arrive at the lab more than 48 hours after time of collection, freeze at -20°C or colder, and ship overnight on dry ice.
- Additional DSHS Laboratory specimen shipping guidelines online here.
|
| Method |
Enzyme-Linked Immunosorbent Assay (ELISA) |
| Turn-around Time |
7 working days
|
| Interferences/Limitations |
- Cross-reactivity with other flaviviruses is very common. Sera from patients infected with Japanese Encephalitis, Wet Nile, and/or Saint Louis viruses may give false positive results.
- Repeated freezing and thawing of samples can cause antibody deterioration.
|
| Common Causes for Rejection |
- Insufficient quantity
- Unacceptable specimen type or source
- Improper shipping conditions
- Expired collection container
- Discrepancies between specimen label and submission form
- Incomplete, illegible, or missing submission form.
|
| Additional Information |
- Reactive samples will be forwarded to CDC for confirmatory testing by Plague Reduction Neutralization Test (PRNT).
- Minimum volume for CDC confirmation testing 0.5 mL.
|