Laboratory Fee Schedule
Procedure: BTA0201
CPT: 83789
Synonym(s): |
Clostridium botulinum toxin, Botulism |
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Requisition Form |
G-27A |
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Test Description |
Detection of Clostridium botulinum toxin |
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Pre-Approval Needed |
- DSHS Epidemiology consultation with physician required
- Must notify BioThreat Team prior to sample submission: (512) 689-5537
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Supplemental Information Required |
Patient history |
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Supplemental Form(s) |
N/A |
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Performed on Specimens from (sources) |
- Human
- Environmental
- Food (associated with a positive clinical sample)
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Sample/Specimen Type for Testing |
- Anaerobic isolate
- Stool
- Gastric
- Water enema
- Serum (no serum on infants)
- Wound swab
- Tissue
- Food (associated with a positive clinical sample)
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Minimum Volume/Size Required |
Feces
- Infant 10 g or 5 mL enema
- Adult 50 g or 5 ml enema
Vomitus or gastric aspirate
Serum (no serum on infants)
Food
Wound swab
Tissue
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Storage/Preservation Prior to Shipping |
Wound swab, anaerobic culture or tissue
- Maintain at ambient temperature
Serum
- Maintain at 2° to 8°C (refrigerated) unless >48 hours, ship at -20°C (frozen)
All others
- Maintain at 2° to 8°C (refrigerated)
Food
- At existing storage temperature, collected by sanitarian only
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Transport Medium |
Isolates sent in anaerobic transport media |
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Specimen Labeling |
Human
- Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)
- Identifiers on specimen must exactly match submission form.
Environmental or Food
- Sample identification must match submission form.
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Shipping and Specimen Handling Requirements |
- Ship according to Dangerous Good Regulations, IATA, and/or CFR 49.
- Handled as infectious agent using universal precautions.
- Triple contained in accordance with federal shipping regulations for infectious agents.
- See also Emergency Preparedness resources online.
- Notify BioThreat Team prior to sample submission: (512) 689-5537
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Method |
Mass spectrometry |
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Turn-around Time |
7-14 days |
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Interferences/Limitations |
- Improper storage or shipping temperatures may affect toxin detection.
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Common Causes for Rejection |
- Insufficient quantity
- Unacceptable specimen type or source
- Improper shipping conditions
- Expired media or collection container
- Discrepancies between specimen label and submission form
- Incomplete or missing submission form
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Additional Information |
May be paired with Culture: Botulinum (human) and/or PCR – Botulinum toxin genes |
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