AFBhplc

HPLC Identification of clinical isolates

G-MYCO

Mycolic acid analysis identification of mycobacterial isolates cultured from clinical specimens in DSHS Laboratory

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Pure isolate from human sources

Mycobacterium species recovered by DSHS laboratory in liquid or on solid culture medium.

• Liquid culture- 2.0 mL
• Solid culture- Single mature colony

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• Two patient-specific identifiers required (e.g., patient full name, date of birth, Medical record number)

• Identifiers on specimen must exactly match submission form.

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High performance liquid chromatography

2 days

• HPLC may only detect the predominant organism in a mixed culture.
• Liquid cultures must be instrument positive in order to analyze by HPLC.
• Additional testing methods may need to be used to provide definitive identification.

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This test is used in-house to identify Mycobacterium species cultured from submitted clinical specimens.